Inhibition of protein kinase p38 increases the differentiation potential of embryonic pluripotent stem cells to endoderm
Human embryonic stem cells, due to the fact that they have the ability to differentiate into all types of cells in the human body, are considered a suitable tool for studying the early stages of human embryo development and differentiation of its cells.
According to the public relations report of Jihad University ،With the aim of knowing the effect of inhibition of p38 protein kinase in the differentiation of human embryonic pluripotent stem cells, Dr. Hossein Baharond, Dr. Seyedah Nafiseh Hosni, Dr. Adele Tai and their colleagues at Royan Research Institute, University of Science and Culture and Max Planck Institute, Germany, during a research on protein inhibition mentioned in embryonic pluripotent stem cells in the absence of fibroblast growth factor.
The results of this research, published in the international journal Biochemical and Biophysical Research Communications, showed that the inhibition of protein kinase p38 leads to an increase in the growth capacity of cells. Also, the analysis of gene expression showed that inhibiting the mentioned protein increases the expression of genes related to pluripotency (OCT4, NANOG, DPPA3, KLF2, KLF5, TBX3). In addition, it was shown that after the inhibition of protein kinase p38, the spontaneous differentiation of human embryonic pluripotent cells will lead to the production of endodermal cells (the innermost layer of the human embryo). The results of this experiment revealed that embryonic stem cells in which p38 is inhibited differentiate into pancreatic progenitors better than normal cells.
The results of this research suggested a new approach for differentiating human pluripotent stem cells into endodermal cells and showed that the resulting endodermal cells have more potential to differentiate into endodermal cells such as pancreas.
Translator:Nosrati
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